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Research Article
Vol. 38, Issue 2
Purification and Characterization of Digitaria exilis Protease (White Acha)
The protease of malted Digitaria exilis (white acha) species was extracted, purified and characterized. The enzyme was extracted with 200 ml of 0.1M citrate phosphate buffer (pH 7) containing 0.4% (w/v) cysteine and 0.86% NaCl, purified by dialysis against 4M sucrose, Ion exchange chromatography on CM sepharose and gel filtration chromatography ...
