Volume 37 (2023)

1

Research Article

The Effect of Aspartame on L-asparaginase Activity using Streptomyces species

Dimowo C. G^*, Osazuwa N. I.

L-asparaginase is the first therapeutic enzyme with antineoplastic properties that has been studied broadly by researchers and scientists far and wide. Aspartame is a methyl ester of aspartic acid and phenylalanine dipeptides. The aim of this research was to ascertain the effect of aspartame on L- asparaginase (enzyme) activities from marine water Isolate. Soil and water samples from lagoon and oc...

Dec, 2023 pp. 6760-6765

2

Research Article

Dynamics of fungi associated with storage of dried fermented cocoa beans varieties in Nigeria

Ogundeji B. A^*, Akintokun, A. K., Akintokun, P. O., Afolabi O. R, Ogundeji F. O, Agbeniyi S. O

Fungi associated with seed coats and nibs of dried fermented F3 Amazon and CRIN Tc series cocoa beans varieties stored for 120 days at 29-34oC/RH 55-65% (low humidity condition) and 29-31oC/RH 85-95% (high humidity condition) were isolated and identified at 15-day intervals (till the end of storage) using standard methods. Percentage of occurrence of each of the isolated fungal species was also ca...

Dec, 2023 pp. 6752-6759

3

Research Article

Isolation and Screening of Linamarase Producing Microorganisms for Detoxification of Cyanide in Cassava

Benjamin C. I^*, Braide, Wesley, Okorondu S. I., Mike-Anosike E. E.

This research focuses on isolation and screening of linamarase producing microorganisms for detoxification of cyanide in cassava tubers. Linamarase is the enzyme that breaks down the toxic substance, linamarin (cyanogenic glucoside) in cassava. A total of six microorganisms were isolated and screened. Out of the six isolates, two were able to grow in a medium containing potassium cyanide (KCN) sol...

Dec, 2023 pp. 6744-6751

4

Research Article

Microbiological Safety of Ready-To-Eat Foods and Hand Hygiene Assessment of Food Handlers in a Nigerian Private University

Oyedeji B. A^*, Bejide O. S, Taiwo M. O, Shofunde A. A, Omeonu F. C, Babalola C. P.

Ready-to-eat foods are widely available in public places including tertiary institutions. The safety of the foods is often compromised by poor hygiene and inadequate sanitary facilities and can have debilitating effects on human health. Unfortunately, the microbiological quality of foods sold at Nigerian universities is rarely routinely assessed. Food samples from the cafeteria of a Nigerian priva...

Dec, 2023 pp. 6736-6743

5

Research Article

Antibacterial Activities of Fenugreek Oil and Seed Extracts on Selected Pathogenic Bacteria and Proximate Composition of Fenugreek Seed

Raji-Idowu F. O. O.^*

Fenugreek is an annually globally grown medicinal plant known for its various bioactive components, including antibacterial activities. It have been consumed medically for food as condiments. The proximate analysis of fenugreek seed showed that carbohydrates (37.79 %) had the highest value followed by fibre (22.48%), protein (20.76%), moisture (8.96%), fat (6.57%) and lowest value was recorded for...

Dec, 2023 pp. 6729-6735

6

Research Article

Antibiotics Resistance: A Global Health Challenge

Aniashi S. O^*, Dike O. I

Infectious diseases have been the leading cause of morbidity and mortality worldwide, particularly in low-income countries, especially in children. The development of antibiotics led to the improvement of life expectancy by about 8 years as they could be used to treat infections that were previously life-threatening. They have also made possible complex surgeries that could otherwise kill the pati...

Dec, 2023 pp. 6721-6728

7

Research Article

Antibacterial Activity of Anacardium occidentale (Cashew) Stem Bark against Bacterial Isolates

Baba, J.^*, Mabekoje O. O., Shehu S, Mohammed S. B, Majiya, H. M, Chock, J.J, AbdulRahaman A. A, Abdullahi, M, Jibril F. L, Dauda, D

The aim of this study was to investigate the relative antibacterial activity and phytochemical properties of ethanolic and water extracts of Anacardium occidentale (cashew) stem bark. The phytochemicals were screened using qualitative methods. Qualitatively analyzed phytochemical constituents in the stem bark extracts includes carbohydrates, alkaloids, flavonoids, saponins, tannins, sterols, anthr...

Dec, 2023 pp. 6712-6720

8

Research Article

Characterization of Antibiotics Resistant Enterococci Isolated from Epe Dam Water, Lagos Nigeria

Oyeyipo F.M^*, Adebanjo A. U, Oyetade O. E, Olanrewaju V. A., Ogundipe E. I.

Dams have been constructed worldwide to dependably provide people with safe water to drink, which has become the practice in developed areas of Nigeria and is acknowledged as a fundamental human right and a cost effective measure for controlling disease. However, the glo bal scientific community is increasingly concerned by descriptions of these dams to be aquatic reservoirs of antibiotic resistan...

Dec, 2023 pp. 6691-6700

9

Research Article

Effect of Different Rates of Single Super Phosphate and Frequency of Megagreen on Nodulation, Nitrogen Fixation and Yield of Cowpea

Adigun M. O.^*, Abiola I. O., Babalola O. A

Megagreen is a bio stimulant, containing micronized calcite whose particles act quickly on the vegetal metabolism via foliar surface. Therefore experiment was carried out at the screen house of the College of Plant Science and Crop Production at the Federal Univer sity of Agriculture, Abeokuta, Ogun State, Southwestern Nigeria to investigate the effect of different rates of single super phosphate ...

Dec, 2023 pp. 6669-6675

10

Research Article

Fermentation and Biocontrol of Mycotoxins in Sorghum bicolor L. Moench Sold in Selected Markets in Abeokuta Metropolis, Ogun State, Nigeria.

Adewunmi A. A^*, Chingoma G. I.

Sorghum bicolor L. Moench (Guinea corn) is a cereal that is widely planted and consumed as staple in Nigeria. Mycotoxin contamination of this grain can present public health concerns to consumers especially children, where it can be fatal. This study investigated the use of fermentation as a biocontrol strategy to mitigate mycotoxins present in Sorghum bicolor grains. A total of 3 0 samples of sor...

Dec, 2023 pp. 6676-6682

11

Research Article

Antimicrobial Activity of Clay against Some Skin Infection Isolates

Isichei-ukah O. B^*, Ofie O. G.

The rising global threat of resistance of microorganisms to antibiotics is alarming. This has necessitated the use of alternative antimicrobial agents in the treatment of infectious diseases caused by pathogens. Clay has since been under investigation for its antimicrobial and therapeutic properties. This study investigated the physicochemical quality and antimicrobial activity of some clay agains...

Dec, 2023 pp. 6683-6690

12

Research Article

Molecular Characterisation and Antimicrobial Susceptibility Pattern of Pseudomonas Species Isolated from Infected Wounds of Patients in Insurgency-Stricken Maiduguri, Borno State

Issa A^*, Ngoshe I. Y, Isa T., Bello S. H., Tom I. M, Benisheikh A., Ali K. H, Edumuyideen I. O, Fowora M. A., Audu R

Multidrug resistant Pseudomonas aeruginosa has been known to cause infections among hospitalised patients leading to significant morbidity and mortality. Carbapenems are usually deployed for treating these infections. This work focused on molecular characterisation and antimicrobial susceptibility pattern of wound infections due to Pseudomonas species from four major hospitals within Maiduguri, Ni...

Dec, 2023 pp. 6701-6712

13

Research Article

Incidence of Neisseria Gonorrhoeae among Patients attending Selected Hospitals in Minna, Nigeria

Baba, J.^*, Mabekoje O. O., Sanda I., Majiya, H. M, Baba U. A, Chock, J.J, AbdulRahaman A. A, Abdullahi, M, Jibril F. L, Dauda, D

Gonorrhoea is a sexually transmitted disease (STD) caused by infection with the Neisseria gonorrhoeae bacterium. This work was carried out from April to June, 2022 to determine the incidence of Neisseria gonorrhoeae among patients attending some selected Hospitals in Minna, Niger State. One hundred and twenty (120) samples collected, which includes, high vaginal swabs,81 (67.50%) , endo cervical s...

Dec, 2023 pp. 6659-6668

14

Research Article

Molecular Characterization of Extended Spectrum Beta lactamase Producing Enterobacteriaceae Isolated from Duck Droppings

Romiluyi B^*, Itaman, V. O., Odumosu B, Adeleye I. A, Smith S. I

The transmission of the members of the Enterobacteriaceae family with extended spectrum has become a cause for concern. Food animals have been researched to be reservoirs of antimicrobial resistance especially in developing part of the world where there is indiscriminate prophylactic use of antibiotics in animal farming. This study investigated molecular identification and antibiotics resistance o...

Dec, 2023 pp. 6652-6658

15

Research Article

Antibacterial Susceptibility Pattern of Bacteria Associated With Wound Infections in Benue State University Teaching Hospital Makurdi, Nigeria

Aernan P. T.^*, Yaji M. E., Obiagwu V. E

Data on the isolated bacteria causing wound infections is currently needed in Nigeria to determine the best management practice and antibiotics to be adopted in wound infection treatment to reduce the cost, pains and improve recovery of affected patients. This work is aimed at identifying bacteria isolated from wound infections in Benue State Teaching Hospital Makurdi Nigeria. Samples were collect...

Dec, 2023 pp. 6643-6651

16

Research Article

Antibiogram of Enteric Bacteria in Pharmaceutical Industrial Wastewater and Surrounding Groundwater Sources

Olalemi, A. O^*, Ajayi, O. J, Abiola, O. O, Usifo, J. I.

This study set out to determine the influence of microbial quality of wastewater from a pharmaceutical industry on surrounding groundwater sources and antibiotic susceptibility of isolated bacteria. Wastewater and water samples were collected from the study sites and the concentrations of enteric bacteria were determined using standard microbiological technique. Physicochemical characteristics of ...

Dec, 2023 pp. 6632-6642

17

Research Article

Phytochemical Screening and Antibacterial Activity of Two Plant Extracts against Multidrug-Resistant Stenotrophomonas maltophilia of Clinical Origin

Agu, G. C^*, Agbolade, O. A, Ezima, E. N, Sossou, I. T, Afolabi, O. T., Onabanjo, M. A, Coker, M. O

New antibacterial agents are pressingly in need to combat the outgrowing incidence of bacterial resistance, which has been number one significant public health threat. The antibacterial activity and phytochemical components of Cannabis sativa and Garcinia kola against Stenotrophomonas maltophilia from diabetic patients with wound infection were investigated. Pure and type isolates of S. maltophili...

Dec, 2023 pp. 6619-6631

18

Research Article

Antibiotics-resistance pattern of bacteria isolated from fish ponds in Ikorodu, Lagos State, Nigeria

Aina, O.R^*, Olaleye, O.N.

Aquacultures are practised in different water confinements which include plastic ponds, concrete and earthen and are known to harbour pathogens. In aquaculture (fish rearing) the use of antibiotics is on the increase where they are used as disease eradicators and as growth promoters. This study sought to investigate the antibiotic resistance pattern of bacteria isolated from fish ponds in Ikorodu,...

Dec, 2023 pp. 6609-6618

19

Research Article

Phytochemical Screening and in –vitro Antimicrobial Activity of Monodora myristica Seed Extract on Selected Human Pathogens

Whiliki O. O^*, Dowe E, Otue E. I.

Monodora myristica is a spice whose various parts are used traditionally for the treatment of a variety of ailments. The purpose of this study was to determine the phytochemical constituents and in-vitro antimicrobial activity of methanolic seed extract of M. myristica against Staphylococcus aureus, Esherichia coli and Candida albicans. Plant seeds were collected, dehulled, air-dried, blended and ...

Jun, 2023 pp. 6599-6608

20

Research Article

Beta-glucosidase Production by Aspergillus niger using Breadfruit Seed Hull as Substrate under a Solid State Fermentation

Osaro-Matthew, R. C^*, Ughala, E, Emmanuel, G. O

Cost effective production of β-glucosidase which is of high value in most biotechnological processes requires an efficient microbial producer, technologies and biomass resources that are economical. This study aimed at evaluating the biochemical characteristics of β-glucosidase produced by Aspergillus niger under solid state fermentation using agro-industrial residues. Fungal isolates from spoilt ...

Jun, 2023 pp. 6588-6598

21

Research Article

Fermented Rice Water for Biosynthesis of Silver Nanoparticles (AgNPs) and its Antimicrobial Activities against Microorganisms Associated with Skin Infection

Yusuf-Salihu, B.O^*, Abdulmumini S. A, Ajao, A.T.

As a skin treatment, rice water is becoming increasingly popular. It is said to aid in the treatment of a variety of skin ailments. Despite the fact that rice water has some genuine benefits, science has yet to fully validate many of its claims. The aim of this study is to biosynthesize nanoparticles using fermented rice water and to carry out the antimicrobial activity of the synthesized nanopart...

Jun, 2023 pp. 6578-6587

22

Research Article

A Review on Clinical Manifestations, Diagnosis and Treatment of Candidiasis among Immunosuppressed Pulmonary Tuberculosis Individuals

Sule, H^*, Kumurya, A. S., Yusha’u, M

Immunocompromized individuals are subject to various forms of infections including that due to opportunistic pathogens like Candida species (candidiasis), which exhibits variety of manifestations seen in different parts of the body of the affected individual. The review aimed to highlight on the occurrence of candidiasis among immunocompromized individuals like TB patients that show various manife...

Jun, 2023 pp. 6570-6576

23

Research Article

Phytochemical Screening and Antibacterial Activities of Ethanolic and Aqueous Leaf Extracts of Alchornea cordifolia and Sida acuta on Organisms Isolated from Meat

Edward K.C^*, Chijioke, D.U., Friday, C.G.

The ethanolic and aqueous extracts of Alchornea cordifolia and Sida acuta were screened for antibacterial activities against isolates from meat. A mortar and pestle were used to macerate the meat and a serial dilution was obtained. The diluents were cultured and biochemical tests were done. For the antibacterial activities, the disc diffusion method was employed while the macro broth dilution meth...

Jun, 2023 pp. 6558-6569

24

Research Article

Human Papillomavirus among Nigerian Women: An Overview

Jabaka, R.D^*, Kuta, F.A, Adabara, N.U, Shittu, K.O

Human Papillomavirus (HPV), is a DNA virus from the family Papillomaviridae (De, Sanjosé et al., 2018 and Virus Taxonomy, 2018). Capable of causing abnormal tissue growth, leading to warts and other changes to cells; thus, it is the major cause of cervical cancers globally. In Nigeria, the increase in the rate of cervical cancer infections and associated deaths is alarming. Thus, the present revie...

Jun, 2023 pp. 6548-6557

25

Research Article

Production of Fermented Cassava Flour (Lafun) using Lactic Acid Bacteria as Starter Culture

Abimbola, O. G.^*, Adedoyin B. A, Ogunremi, O. R, Adeyemo, S.M

Lafun, a fermented cassava product, confronts challenges in terms of product quality and safety as a result of spontaneous fermentation systems. To resolve these issues, a production method utilizing starter cultures was developed. Lactic acid bacteria were isolated from fermented cassava and screened for their technological properties. The physicochemical parameters, proximate and antinutrient co...

Jun, 2023 pp. 6536-6547

26

Research Article

Microbiological, Nutritional and Organoleptic Changes Associated with Fermented Oil Bean (Pentaclethra macrophylla Benth) Seed (ugba) Stored at Ambient Temperature

Obi, C. N.^*, Oriaku, C. P, Ademe, O. F., Epundu, I. B., Okore, C. O.

Ugba is the Igbo name of the fermented African Oil bean seeds (Pentaclethra macrophylla Benth). It is a traditional food condiment generally produced by natural fermentation and is an important and cheap source of protein for people whose staple foods are deficient in protein. The study was carried out to determine the microbiological, nutritional and organoleptic changes associate with ugba when ...

Jun, 2023 pp. 6523-6535

27

Research Article

Effect of Fermentation Method on Nutritional, Anti-Nutritional Composition and Microbial Properties of Mung Bean (Vigna radiata) 'Iru'

Lawal, R.T^*, Fabiyi, K, Aboderin, E.A, Adetuberu, I.A, Ajayi, A.N.

The study investigated the effect of fermentation on the nutritional and anti-nutritional composition of fermented mung bean ‘iru’ as a protein condiment. The mung bean was processed to mung bean ‘iru’ using the local method of producing ‘iru’ from African locust beans. The mung bean was sorted, washed and boiled for 1 hr. The boiled seeds was dehulled to remove the seeds coat, washed and boiled a...

Jun, 2023 pp. 6517-6522

28

Research Article

Bacteriological Quality and the Antibiogram of Isolates from Raw Cow Milk Produced in Ibadan Metropolis Oyo State, Nigeria

Adediran A.B^*, Aforijiku, S, Adediran A.T, Fashogbon, R. O

This study aimed at evaluating the bacteriological quality as well as the antibiogram (antibiotic sensitivity profile) of organisms isolated from raw cow milk produced in Ibadan metropolis. Raw cow milk samples were collected from University of Ibadan Research farm, Bodija, Sabo and Akinyele cow settlement ibadan. Bacterial counts, isolation and phenotypic identification of bacterial isolated from...

Jun, 2023 pp. 6508-6516

29

Research Article

Fungal Laccase Production; a Tool for Biodegradation of Maize Cobs

Olagunju, A.^*, Onyike, E., Ameh, D. A, Atawodi, S. E.

Laccases are increasingly being used in the biodegradation and utilization of biomass. This trend has led to increased need for development of efficient production systems. This research was carried out with the aim of optimizing the fermentation conditions of selected and consortia of four fungal species (Lachnocladium flavidum, Aspergillus niger, Trichoderma reesei, and Lenzites betulina) for pr...

Jun, 2023 pp. 6477-6486

30

Research Article

Production and Proximate Composition of Yoghurts from Tiger nuts and Soybean using Lactic Acid Bacteria Starter Cultures

Obi, C. N.^*, Chiekie, U. I, Oriaku, C. P, Ogele, C. P.

Traditionally, yoghurt is fermented whole milk. It is believed to possess nutritional and therapeutic properties. Appropriate aliquot of commercially prepared yoghurts was sourced and used to isolate LAB bacteria using standard procedures. The morphological characteristics of the isolates were studied and recorded. They were later stored in MRS agar slants at 4oC in the refrigerator for further us...

Jun, 2023 pp. 6487-6498

31

Research Article

Seroprevalence and Associated Risk Factors of Toxoplasma gondii among Pregnant Women Attending Ante Natal Clinics in Zaria, Kaduna State, Nigeria

Adamu, S^*, Doko M.H.I, Inabo H. I

Toxoplasmosis is a parasitic disease caused by Toxoplasma gondii an obligate intracellular parasite. It results in severe complications in the foetus if the infection is acquired during pregnancy including spontaneous abortion, low birth weight, congenital malformations and intrauterine death. The study was Hospital based and cross-sectional and was carried out among pregnant women attending anten...

Jun, 2023 pp. 6499-6507

32

Research Article

Incidence of Bacteria Associated with Post Harvest Rot of Colocasia esculenta LIN (Cocoyam) Tubers Sold in Different Markets in Bukuru, Jos South Local Government Area, Nigeria

Danahap, L. S.^*, Christopher, I, Okechalu, O. B.

The bacteria species associated with post-harvest rot of Colocasia esculenta Lin (Cocoyam) tubers sold in Bukuru market of Plateau State were investigated. Fifty (50) Samples of C. esculenta were obtained randomly from 3 different Markets within Bukuru and brought for analysis. The tubers were surface disinfect with Sodium Hypo chloride (NaOCl) for 5 minutes. A portion of the rotten sample and the...

Jun, 2023 pp. 6469-6476

33

Research Article

Impact of Pesticides on Microbial Population in LASUSTECH Agricultural Field, Nigeria

Aina, O.R^*, Olaleye, O.N.

Pesticides are used to increase crop production. Besides combating pests, pesticides affect the activity of beneficial soil microbial communities thereby affecting the nutritional quality of soils. This study investigated the impact of pesticides on the ecological activity of microorganisms in LASUSTECH agricultural field. Twenty milliliters of Fungicides (Copper (1) oxide metalyxl and 2,3-Dichlor...

Jun, 2023 pp. 6459-6468

34

Research Article

Cultural and Molecular Characterization of Bacterial Contaminants from Pounded Yam Sold Along Major Roads in Makurdi Metropolis, Benue State, Nigeria

Womboh, S. B^*, Ajumobi, V. E, Ebute, P. A, Iheukwumere, C. C.

Roadside foods are often contaminated by various pathogenic bacteria, which can cause food borne diseases. This study was aimed at using molecular methods to identify bacterial contaminants in pounded yam sold along major roads in Makurdi metropolis, Benue State, Nigeria. Isolation and biochemical characterization of the bacterial contaminants in pounded yam samples was done on Nutrient agar, Eosi...

Jun, 2023 pp. 6451-6458

35

Research Article

Antibiogram of Clinical Isolates of Escherichia coli and Klebsiella species Producing Extended Spectrum Beta Lactamase (ESBL)

Ovia, K. N^*, Ibiam, U, Okoh, N, Okafor, C, Eromonsele, B, Okoroafor, I, Ejikeugwu, C

The public health threat posed by bacteria producing extended spectrum beta lactamase (ESBL) is increasingly contributing to the global challenge of antimicrobial resistance (AMR) which is slowly ushering in a post antibiotic era – where no antibiotic is likely to work. AMR is a global health threat that warrant urgent steps to mitigate its growing ugly trend. This study investigated the susceptib...

Jun, 2023 pp. 6442-6450

36

Research Article

Aflatoxin Contamination in Spices Sold in Ilorin, North-Central, Nigeria

Ahmed El-Imam^*, Amina, M, Oguntimehin, S. A, Sanni, A.

Spices are commonly contaminated with aflatoxins, a group of potent mycotoxins produced by moulds. This study investigated the incidence of aflatoxigenic fungi and aflatoxin contents in spices commonly used in Ilorin, North Central Nigeria. Sixty samples of thirteen different spices were purchased from three markets. Fungal isolates were identified using phenotypic characteristics. Aflatoxin conta...

Jun, 2023 pp. 6424-6431

37

Research Article

Bacteriuria Among Diabetic and Non-diabetic Patients in some Hospitals in Rivers State, Nigeria

Hanson, A. H^*, Akani, N.P, Williams, J. O., Sampson, T

Among the great diversity of etiological agents attributed to urinary tract infections, bacteria are the major causative organisms that are responsible for more than 95% of Urinary Tract Infections ( UTIs). This study aimed at the isolation and identification of bacteria in urine from diabetics and non-diabetics. A total of 300 mid-stream early morning urine specimens were collected from diabetic ...

Jun, 2023 pp. 6432-6441