Volume 38, Issue 1 (June)

1

Research Article

Microbial Impact Assessment of a Municipal Abattoir Operations on Adjoining Soils of its Receiving Water Milieu

Ayeni T. O^*, Olusola-Makinde O. O, Arotupin Daniel Juwon

Wastewater from abattoirs have been documented to have harmful impact on the surrounding soil environments. This study therefore, assessed soil samples for possible abattoir wastewater contamination of physicochemical parameters and microbial composition from a mini abattoir in South Local Government Area of Ondo State, Nigeria. Soil samples were collected from the soil 2 m away from abattoir site...

Jun, 2024 pp. 6788-6797

2

Research Article

Isolation and Identification of Non Dermatophytic Moulds from Different Air and Soil Samples from Cattle Markets in Abia and Imo State, Nigeria

Nwofor C. N^*, Echeta, M. O, Onyenwe, N. E, Oyeka, C. A

The prevalence of non-dermatophytic moulds from air and soil samples from six cattle markets in Abia and Imo state, Nigeria were carried out simultaneously. A total of 60 (sixty) samples of air and soil were analysed. The settlement plate technique, tube dilution method and hair baiting technique were evaluated for the air and soil for the presence of keratinophilic fungi. Different species of 13 ...

Jun, 2024 pp. 6798-6808

3

Research Article

Biogenic Synthesis of Silver Oxide Nanoparticle from Plants Materials for Control and Management of Microbial Infections in Fishes

Ekaji F. A.^*, Ogbulie J.N, Braide, Wesley, Akujobi C. O, Ajima M

Nanoparticles has shown to be effective in drug delivery, though, its synthesis are time and energy consuming while, the process is toxic to the environment, hence, the utilization of green synthesis method with plants extracts. The study focused on the green synthesis of nanoparticles and its application on isolates of diseased fish. Four different isolates namely; Bacillus infantis, Klebsiella p...

Jun, 2024 pp. 6809-6816

4

Research Article

Molecular Characteristics, Antibiotic Sensitivity, and Substrate Specificity of Biosurfactant-Producing Bacterial Isolates from Crude Oil Spill Sites in Niger Delta: A Comprehensive Review

Emeanuru P. C^*, Ogbulie J.N, Adieze I. E., Nwachukwu, I.N., Braide, Wesley, Nwosu C. J

The Niger Delta area, renowned for its biodiversity, has suffered severe environmental degradation due to crude oil spills resulting from oil exploration and exploitation activities. To address this, researchers have explored biosurfactant-producing bacteria as eco-friendly solutions for remediating polluted sites. Indigenous strains, such as Pseudomonas aeruginosa and Bacillus subtilis, have been...

Jun, 2024 pp. 6817-6828

5

Research Article

Assessment of Electromagnetic Field on Bacteriological and Physicochemical Properties of Brewery Effluent from Ilesha

Balogun O. B^*, Akinyosoye F. A, Arotupin Daniel Juwon

The present study was designed to enumerate and identify microorganisms and to determine physicochemical properties of industrial effluents. Samples were collected from four different industries in Oriade local government (Ilesha, Ikeji-Arakeji and Ipetu Ijesha). The Industrial effluent samples were subjected to microbiological and physicochemical analyses. Bacteria isolated from industrial efflue...

Jun, 2024 pp. 6828-6837

6

Research Article

Screening and Identification of Biosurfactant-Producing Bactria from Crude Oil Contaminated Soil and Water in the Niger Delta Area, Nigeria

Emeanuru P. C^*, Ogbulie J.N, Adieze, Ifechukwu E., Nwachukwu, I.N., Braide, Wesley

Crude oil pollution is sine qua non in oil exploration and exploitation activities. Biosurfactant increases bioavailability and help to enhance contact between the pollutants and the microorganisms and therefore facilitates uptake and degradation as well as amelioration (remediation) of hydrocarbon polluted environment. Biosurfactant is preferred to chemical surfactant in crude oil remediation. Th...

Jun, 2024 pp. 6838-6846

7

Research Article

Degradation of Diesel by Staphylococcus sciuri Strain XB1 Isolated from a Dumpsite in Lagos State, Nigeria

Fashola M. O.^*, Ashade A. O, Opere, B. O., Dawodu T. B, Anagun O. S., Semako S. D, Obayori, O. S

Staphylococcus sciuri strain XB1, a diesel degrading bacterium, was isolated from Igando dumpsite by continuous enrichment technique on diesel mineral salt medium (MSM). The isolate was identified using cultural, morphological, biochemical characteristics and complemented with analytical profile index. The isolate is yellowish, Gram-positive cocci, catalase positive, oxidase and coagulase negative...

Jun, 2024 pp. 6847-6858

8

Research Article

Fungal Contaminants of Feed, Litter and Faecal Dropping Samples of Poultry Birds, their Occurrence and Distribution in Selected Farms in Anambra State, Nigeria

Mba A. N^*, Ekwealor C. C., Ekwealor I. A.

In Nigeria, fungi have been causing high mortality and production loses in the poultry industry. This study aims at quantifying the fungal organisms in feed, litter and birds droppings, and their occurrence and distribution in selected poultry farms in Anambra State, Nigeria. The samples were collected from 10 poultry farms in six local government areas in Anambra State, Nigeria. One gram (1 g) of...

Jun, 2024 pp. 6858-6870

9

Research Article

Assessment of Proximate and Antioxidants Profile oF Pleurotus ostreatus (Jacq) P. Kumm. Cultivated on Selected Medicinal Plant Leaves

Ayo E. O^*, Olalemi A. O., Arotupin Daniel Juwon

Oyster mushrooms can synthesize great number of minerals, and secondary metabolites that possess antioxidant activities which play important role in maintaining human health. This study investigated the proximate, mineral and antioxidant properties of Pleurotus ostreatus cultivated on medicinal leaves such as Neem leaves (Azadirachta indica), Moringa leaves (Moringa oleifera), Lemon grass (Cymbopo...

Jun, 2024 pp. 6871-6884

10

Research Article

Microbiological Examination of Personal Effects of Undergraduate Students in Microbiology Laboratory

Ofon U. A.^*, Okon, M. U, Udofia E. S., George M. U

Personal effects (PEs) including phones, laptops and bags globally are a necessary part of our everyday lives. These PEs are often carried or worn by individuals for numerous purposes, globally. However, the increasing risk of microbial contamination and the presence of antibiotic-resistant strains on these devices are of public health significance. Using standard microbiological procedures, this ...

Jun, 2024 pp. 6885-6894

11

Research Article

Cultivation of Pleurotus ostreatus (Oyster Mushroom) Using Sawdust Supplemented with Waste Human Hair

Obuekwe I. S^*, Odemwingie O. S.

Waste human hair (WHH) is a part of municipal solid waste generated from salons that may lead to clogging of drainage pipes ensued with flooding. Pleurotus ostreatus (mushroom) is capable of utilizing several organic substrate types due to its enzyme secretions. This study determined the potential of WHH in cultivating edible mushrooms P. ostreatus (Oyster mushroom).The WHH from males was collecte...

Jun, 2024 pp. 6895-6905

12

Research Article

Toxicological Effects of Electronic Waste (E-waste) on Microbial Flora and Radionuclides

Ocheoibo S. J^*, Atuanya E. I

This study was conducted to investigate the microbial, elemental levels and radionuclei concentration of three e-waste dumpsites with a view to establish the contamination status of these sites. E-waste soil samples were collected from Oluku and Osasogie in Benin, Edo state Nigeria while the third E-waste soil sample was obtained from Alaba, Lagos state Nigeria.The microbial analysis was done base...

Jun, 2024 pp. 6906-6915

13

Research Article

Antifungal Susceptibility and Evaluation of Risks Associated with Heavy Metals Distributed in Selected Dumpsite Soils of Osogbo Metropolis, Southwest Nigeria

Titilawo M. A^*, Ajani T. F, Akinleye G. O., Ogunlana E. O, Ojediran G. O, Titilawo O. Y., Olaitan J. O

The study assessed the drug susceptibility, ecological and human health risks associated with toxic metal concentrations of five dumpsite soils in Osogbo metropolis, Southwest Nigeria. Fungal distribution and susceptibility, heavy metal (Cd, Cu, Fe, Pb, and Zn) levels of Egbedi, Gbonmi, Ilesa garage, Omobolanle, and Oke-Baale dumpsite soils were determined following standard protocol. Pollution, e...

Jun, 2024 pp. 6916-6929

14

Research Article

Occurrence of Metallo-beta-lactamase in Multidrug Resistant Escherichia coli Isolated from Donkey Slaughter Market

Ugbo E. N^*, Ugbo A. I, Agumah B. N, Nwojiji E. C, Ewah C. M, Ogbonna I. P, Ekpono C. U.

Animals used in food production have been identified as one of the major primary sources of antibiotic resistant pathogenic bacteria within the Enterobacteriaceae family. The Escherichia coli harboring metallo-β-lactamases (MBLs) is a serious threat to global health. This study was aimed at the occurrences of metallo-beta-lactamase in multidrug resistance Escherichia coli isolated from donkey slau...

Jun, 2024 pp. 6930-6939

15

Research Article

Induced Fermentation for the Production of Virgin Coconut Oil (VCO) Using Bacillus cereus Strain ST06 (MH475925.1) and Bacillus velezensis Strain Bv1-4 (OQ787546.1)

Adedayo M.R^*, Kolawole W. O., Ajiboye A. E

The use of bacteria in fermentations to produce functional foods has greatly increased in recent years due to the numerous associated benefits. Moreover, the increased demand for the production of virgin coconut oil (VCO) at household and industrial level as well as the need to overcome the problem of contamination associated with spontaneous fermentation justifies this research. Matured Coconut f...

Jun, 2024 pp. 6940-6946

16

Research Article

Probiotics Potential of Bacteria Associated with Fermented Unripe Plantain (Musa paradisiaca) Juice Sold in Orieugba Market Umuahia, Nigeria

Appeh O. G^*, Ugbogu O. C.

Probiotics are live microorganisms that when administered in adequate amounts, confer a health benefit on the host. They are naturally present in fermented foods, may be added to other food products and are available as dietary supplement. This study was aimed to determine probiotics potentials of bacteria associated with fermented unripe plantain (Musa paradisiaca) juice. The physico-chemical ana...

Jun, 2024 pp. 6947-6953

17

Research Article

Isolation and Characterization of Probiotic Lactic Acid Bacteria from Fermented Foods Using Conventional and Molecular Methods

Hassan M. R^*, Saleh, A. A., Muhhammad B. T.

The study aimed to isolate and characterize probiotic lactic acid bacteria from fermented foods using conventional and molecular methods. The isolation was done using De Man, Rogosa, and Sharpe (MRS) agar using standard procedure and standard biochemical tests for the identification of lactic acid bacteria. The isolates were confirmed using polymerase chain reaction (PCR), sequence analysis and co...

Jun, 2024 pp. 6954-6960

18

Research Article

Molecular Characterization of Bacterial Isolates from Conventionally Stored African Oil Bean Seed in Ilorin Metropolis

Ihum T. A^*, John W. C, Oledibe C. F.

The African oil bean seed (Pentaclethra macrophylla) is widely used as a traditional food condiment and is valued for its high protein content. This study focused on assessing the microbiological quality and molecular characterization of microorganisms associated with African oil bean seeds stored under conventional methods. Samples were obtained from three different markets in the Ilorin metropol...

Jun, 2024 pp. 6961-6968

19

Research Article

Quality Improvement of Watermelon-Clerodendrum volubile Extract Wine Produced via Sequential Malolactic Fermentation by Saccharomyces cerevisiae and Lactobacillus delbrueckii

Kazeem M. O.^*, Aderoboye Y. O., Oyinlola K. A, Maiangwa J

Herbal infusions medicinal benefits in wine and the impact of malolactic fermentation on wine quality is of high significance. The study aimed at improving the quality of watermelon wine with Clerodendrum volubile extract using Saccharomyces cerevisiae and Lactobacillus delbrueckii subsp. bulgaricus. S. cerevisiae and L. delbrueckii isolated from palm wine and yoghurt, respectively were used in th...

Jun, 2024 pp. 6969-6978

20

Research Article

Microbial, Proximate and Sensory Evaluation of Bread Produced from a Blend of Wheat and Corn Flour

Mbachu A. E^*, Otuya W. A, Mbachu N. A

Wheat flour, which is the major raw material used in baking bread, is being imported in Nigeria and other countries where cultivation of wheat is hampered by unfavourable climate. This has necessitated the rising cost of bread beyond the reach of the poor in Nigeria. This study was aimed at assessing the quality of bread produced from a blend of wheat and corn flour. Composite breads were produced...

Jun, 2024 pp. 6979-6986

21

Research Article

Genetic Characterisation and Symbiotic Efficiency of Rhizobia Nodulating Different Landraces of Bambara Groundnut (Vigna subterranea L. Verdc)

Oloyede A. R.^*, Ojo, A. E., Taiwo E. A

The identification of native rhizobia capable of inducing effective nodulation in legumes is a prerequisite for selection of strains for bioinoculation. The study aimed to characterize and evaluate the symbiotic efficiency of native rhizobia capable of effectively nodulating Bambara groundnut (Vigna subterranean). Greenhouse experiment was conducted in a completely randomized design with five acce...

Jun, 2024 pp. 6987-6997

22

Research Article

Street Vended Donkunu (Kenkey) and its Raw Sauce in Southwest Nigeria: A Plethora of Potential Pathogens and Source of Foodborne Illnesses

Oyafajo L. A^*, Aremu H. K, Adeyemi F.M, Oyedokun J, Bello T., Akala A. O, Abiola, O. O

Street vended foods attract the populace because of their savory taste preferred by the indigenous public, and have contributed significantly to the maintenance of the food supply chain. Consumption of street food is significantly increasing due to a busy scheduled life. Donkunu and its complementary sauce is a Ghanaian delicacy that has been consumed in Nigeria over the years, but the safety stat...

Jun, 2024 pp. 6998-7008

23

Research Article

Prevalence of Salmonella spp. in Poultry and Poultry Feeds in Makurdi Benue State, Nigeria

Tyoga K. M^*, Ikpa T. F, Sar T. T.

The study was undertaken to assess the risks of Salmonella infection present in faecal stained eggs, faecal materials and poultry feeds from live poultry markets and poultry feed shops in Makurdi Benue State, Nigeria. A total of 300 samples comprised of 100 faecal stained egg surface washes, 100 faecal materials and 100 poultry feed samples were collected. Enrichment, isolation and identification ...

Jun, 2024 pp. 7009-7013

24

Research Article

Isolation and Identification of Microorganisms from Herbal Mixtures Sold Within, Abuja, Nigeria

Agada E. O.^*, Mohammed S.S.D., Olatun A.

The safety, efficacy and quality of herbal mixtures has become a concern to health authorities and health professionals, especially, with rise in microbial resistance to drugs and the demand herbal mixtures as alternative remedies. Thus, the need to isolate and identify microorganisms from some liquid herbal mixtures sold within Abuja, Nigeria. A total of ten samples of herbal mixture were selecte...

Jun, 2024 pp. 7014-7022

25

Research Article

Determinants of Empirical Antibiotics Candidates for Ear, Nose and Throat Infections in a West African Tertiary Hospital

Ademakinwa O. R^*, Adeyemo A. A, Ayodele S. O, Oluduro A. O

50 participants. A total of 17 different bacterial species were identified from 192 bacteria isolated and 113 (58.9%) bacterial isolates were cultured. Staphylococcus species 62 (32.3%) especially Staphylococcus aureus (21.1%) and Proteus mirabilis 29 (15.1%) were the most prevalent Gram-positive and Gram-negative bacteria respectively. The isolates were more susceptible to vancomycin, meropenem, ...

Jun, 2024 pp. 7023-7031

26

Research Article

Simultaneous Detection of Dengue and West Nile IgM Antibodies in Febrile Individuals in Ile-Ife, Nigeria

Adesina O. A^*, Amoo B. A, Adeboje M. F, Simeon O. N, Yusuf O. O

While there are over 80 members of the Flaviviridae Family, some of which represent a global threat, Dengue, and West Nile viruses represent two of the major human pathogens that produce life threatening conditions and complications. These mosquito-borne viral infections have not so much been studied in rural and sub-urban areas of the sub-Saharan regions where the vectors thrive. This study seeks...

Jun, 2024 pp. 7032-7039

27

Research Article

Drevalence of Impaired Renal Function among Prostate Enlarged Adult Attending General Hospitals in Benue State, Nigeria

Agada E. O.^*, Iwodi C., Ogbonna I. O.

Prevalence of impaired renal function among prostate enlarged adult attending General Hospitals in Benue State, Nigeria was carried out. Three hundred and sixty eight (368) samples of blood was collected from the 23 General Hospitals located in the Local Government Headquarters in Benue State, Nigeria. The plasma were used for the quantitative determination of prostate specific antigen (PSA), crea...

Jun, 2024 pp. 7040-7044

28

Research Article

Characterization of Extended Spectrum Beta-Lactamase Producing Escherichia coli isolates from Swine in Ohaukwu Local Government Area, Ebonyi State Nigeria

Awoke O. O.^*, Iroha I. R, Nwankwo F. M., Ugbo, E. N, Awoke C. U, Edeh P. A., Okpaga J. U, Oke B

Antimicrobial resistant bacterial strains have become a global public health challenge affecting both humans and domestic livestock such as cattle, pork and poultry. This research work focused on the characterization of extended spectrum beta-lactamase producing Escherichia coli isolates from swine at Ohaukwu Local Government Area in Ebonyi State, Nigeria. A total of 400 (200 each of rectal and na...

Jun, 2024 pp. 7045-7058

29

Research Article

Isolation and Identification of Candida auris from Cutaneous Surface of Patients on Long-term Care in Afe Babalola University Multi-System Hospital, Ado-Ekiti, Nigeria

Ayuba S. B^*, Ogwu N. I, Akinseye J. F., Oluboyo B. O, Egbebi A. H, Thomas H, Z

Nearly 150 Candida species have been described and are part of the microbiome on human skin, mucous membranes, the female genital tract, and the gastrointestinal tract, but only 10% of them are known to cause human diseases (candidiasis). The typical human commensal flora contains a number of species of Candida. Recently identified is Candida auris, a multidrug-resistant yeast has emerged as a pro...

Jun, 2024 pp. 7059-7066

30

Research Article

Antibiotic Resistance and Plasmid Profile of Escherichia coli Isolated from Pregnant Women in Abia State

Ngoka D. I.^*, Nwankwo, E.O, Okey-Kalu E. U

This study was undertaken to determine the antibiotic resistance and plasmid profile of Escherichia coli isolated from pregnant women in Umuahia. A total of 250 midstream urine samples from pregnant women were analyzed and cultured on cystein lactose electrolyte deficient (CLED) agar and MacConkey agar. Antibiotic susceptibility test was done by Kirby-Bauer disc diffusion method. Plasmid profiling...

Jun, 2024 pp. 7067-7074

31

Research Article

Prophylactic and Combination Study on Phyllanthus amarus and Diodia scandens Bioactive Fractions against Antibiotic Resistant Staphylococci

OJO S.K.S, Aliu K. T^*, Jeje T. O, Balogun D. M, Orire V. O, Atitebi C. O, Awokoya O. O

Since centuries ago till date, medicinal plants remain relevant for curing many human infections and diseases due to the many enriched therapeutic and bioactive compounds present in plants. The age long tradition of drug discovery has always been aided by natural products from plants; which provide the basic elements for drug discovery. This research aims at evaluating the prophylactic effects of ...

Jun, 2024 pp. 7075-7084